边坡不同深度农业土壤肥力分析

以安新地区高速公路边坡坡面农业土壤为研究对象,通过分层取样的方法,对不同深度土壤的pH、有机质、碱解氮、有效磷和速效钾含量进行对比分析,探讨高速公路对周边不同深度土壤肥力的影响。结果表明:边坡不同深度农业土壤的有机质、碱解氮、有效磷、速效钾含量不同,调查范围内的土壤质地以粉砂质粘壤土为主,以壤质砂土为辅。高速公路对边坡不同深度土壤有机质含量的影响最大,其次是有效磷,影响最小的是速效钾,pH也受边坡不同深度土壤的影响。由此可见,安新地区高速公路能够对边坡不同深度土壤的肥力状况产生一定的影响。     

Study on Anti-viral Effect of Rhubarb on Porcine Epidemic Diarrhea Virus in vitro

The inhibitory effects of rhubarb on cell lesion, which was caused by porcine epidemic diarrhea virus (PEDV), and its mechanism had been studied in this research.The maximum safe concentration of rhubarb solution applied to Vero cells was determined through morphological observation, and then the PEDV infected Vero cell model in vitro was established, by which the inhibitory effect of rhubarb on virus was studied.The experimental method had also been established based on this cell model.The result showed that the maximum safe concentration was 3.28 mg/mL.Both morphological observation and MTT assay were indicated that rhubarb could not only block the attachment of the virus to Vero cell and inhibit the synthesis and reproduction of the virus, but also could directly inactivate the virus.This research showed that rhubarb had an inhibitory effect on PEDV, and provided a theoretical basis for the prevention and treatment of clinical porcine epidemic diarrhea.     

Neuroprotective effect of fasudil combined with bone marrow-derived neural stem cells on mice with experimental autoimmune encephalomyelitis

AIM: To explore the neuroprotective effect of fasudil combined with bone marrow-derived neural stem cells (BM-NSCs) on the mice with experimental autoimmune encephalomyelitis (EAE). METHODS: Female C57BL/6 mice (8~10 weeks old, n=32) were immunized with myelin oligodendrocyte glycoprotein 35-55 (MOG_35-55) to establish chronic EAE model. The mice were randomly divided into control (ddH₂O) group, fasudil group, BM-NSCs group, and fasudil+BM-NSCs group. The clinical score and body weight were recorded every other day. The expression of neurotrophic factors was determined by immunofluorescence staining. RESULTS: In comparison with ddH₂O group, fasudil combined with BM-NSCs delayed onset and ameliorated severity of EAE. The numbers of brain-derived neurotrophic factor, glial cell-derived neurotrophic factor, nerve growth factor, neurotrophin-3 and ciliary neurotrophic factor positive cells in fasudil group, BM-NSCs group and fasudil+BM-NSCs group were all increased in various extents. In particularly, the expression of these neurotrophic factors in fasudil+BM-NSCs group was significantly higher than that in the mice treated with fasudil or BM-NSCs alone (P<0.01). CONCLUSION: Fasudil combined with BM-NSCs promotes the expression of neurotrophic factors and improves microenvironment of central nervous system, thus playing a positive role in neural restoration and regeneration through a synergistic and superimposed effect.     

绒毛白蜡新品种‘华雄’

 ‘华雄’是在白蜡属种质资源收集过程中发现并选育出的绒毛白蜡新品种。雄株，干形较直，当年生枝和纸质叶片上下表面均被密毛，耐盐碱性强。     

不同种源软枣猕猴桃幼苗光合特性的比较及变异分析

以5个种源的软枣猕猴桃2年生幼苗为试材,采用LI-6400便携式光合测定系统测定其光合指标,分析不同种源软枣猕猴桃的光合日变化,揭示种源间光合特性的差异。结果表明:不同种源软枣猕猴桃幼苗净光合速率、气孔导度和水分利用效率日变化呈"双峰"曲线;蒸腾速率有"单峰型"和"双峰型"2种形式;胞间CO_2浓度呈‘W’型趋势;不同种源间存在一定的差异,各时间段的光合指标大小、峰值的大小及出现的时间有所不同。不同种源间的净光合速率、蒸腾速率、气孔导度、胞间CO_2浓度和水分利用效率存在极显著差异,通过隶属函数值综合评价,敦化和林口种源的光合能力强,属于较高光效种源。     

不同播期对鲁原502小麦产量的影响

为实现良种良法配套推广应用、大面积高产,以目前莒县优选的鲁原502小麦作供试材料,对该品种的播期进行研究。结果表明,播期对鲁原502产量的影响显著,其在莒县的适宜播期为10月5—15日。     

葡萄霜霉病菌侵染抗病和感病品种过程的组织学观察

用苯胺蓝染色结合扫描电镜的方法,观察葡萄霜霉病菌(Plasmopara viticola)在葡萄感病品种‘红提’和抗病品种‘北玫’中的侵染过程,发现两品种从接菌到显症的过程基本一致,但孢子囊伸出气孔的时间、伸出孢囊梗气孔比例、孢囊梗数量及孢囊梗分支,‘红提’比‘北玫’多;菌丝在‘北玫’上生长速度慢,后期生长量也较少。‘红提’的潜育期为4 d,而‘北玫’的为5 d,‘红提’和‘北玫’的长菌丝、分支的长菌丝、疏松菌丝体出现的时间也不同。菌丝生长一段时间后,在‘北玫’上停留在疏松菌丝体阶段,而‘红提’96 h时叶片组织内长满致密的菌丝体。两品种在初生菌丝和长菌丝阶段产生吸器的频率差异显著。     

苹果‘长富2号’开花调控转录因子基因MdIDD7的克隆及表达分析

以‘长富2号’苹果为试验材料,采用RT-PCR的方法,从其芽中克隆得到INDETERMINATE DOMAIN(IDD)转录因子基因MdIDD7,其开放阅读框长度为1 626 bp,编码541个氨基酸。序列比对和结构域分析表明,该转录因子含有1个核定位信号和4个高度保守的锌指蛋白结构域。系统进化树分析表明,MdIDD7与白梨(Pyrus×bretschneideri,XP_009364602.1)、桃(Prunus persica,XP_007225628.1)和梅(Prunus mume,XP_008220893.1)聚在一起。实时荧光定量PCR表明,MdIDD7在‘长富2号’不同组织(茎、叶、花、果和芽)中均有表达,其中芽的表达量最高。花后40~60 d,MdIDD7在易成花品种‘烟富6号’芽中表达量显著高于难成花品种‘长富2号’。"小年"树顶芽组织中MdIDD7的表达量显著高于"大年"树。在花芽诱导前期,外源GA处理诱导MdIDD7下调表达,而蔗糖处理诱导其上调表达,说明MdIDD7响应激素和糖信号,促进苹果成花。     

不同滴灌频率对大田覆膜小西瓜生长特性及品质的影响

在灌溉定额一定的情况下,研究了不同滴灌频率对大田膜下小西瓜在果实膨大期以及成熟期内有关特性的影响,以期为豫北平原地区大田膜下滴灌小西瓜生长中后期阶段确定最佳的滴灌频率。结果表明:不同灌溉频率对小西瓜生长过程中根粗、茎粗和蔓长的影响不是特别明显。从试验数据来看,以果实膨大期中频(T3),果实成熟期低频(T5)处理效果较好。此灌溉频率组合下不仅小西瓜长势好,而且甜度较大,省工省时又节水。     

基于模糊数学感官评价和混料设计的低度甜糯玉米酒原料配方优化

为了优化以鲜食甜糯玉米为主要原料制备甜糯玉米酒的原料配方。试验运用模糊数学原理,将甜糯玉米酒感官品质数学抽象量化,以量化后的感官品质为指标,通过混料试验设计,建立原料配方与感官品质之间的回归模型。结果表明:该模型的拟合程度好(R=0.984 4,P0.001),可用于预测和分析甜糯玉米酒的感官品质。经模型优化可得,感官品质评价为"好"的甜糯玉米酒原料配方为鲜食糯玉米(66%)、鲜食甜玉米(17%)和糯米(17%)。本研究酿造的甜糯玉米酒的酒精度仅为4.75%,远低于一般低度酒,符合现代人的饮酒需求。